Brookline is a former village in Greene County, Missouri, United States. The population was 326 at the 2000 census. In 2005, Brookline consolidated with the city of Republic after a vote of the residents of both communities approved the consolidation. The individual names of Brookline and Republic were to be used for each area, but the town would be governed under the city of Republic. Since this time, the city limit signs of Brookline have been removed and replaced with Republic's. Small markers on the main road through Brookline label the main part of the town, though these are ones used by Missouri for unincorporated communities.